Cryogenic Electron Microscopy of Beam and Air-Sensitive Materials
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Cryogenic Electron Microscopy of Beam and Air-Sensitive Materials
Date: September 9, 2021 Time: 11:00am (PDT), 12:00pm (EDT) Advances in cryogenic electron microscopy (cryo-EM), primarily driven by the life sciences, have emerged as powerful techniques for
Sep 9, 2021
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What's cryo-EM? Cryogenic electron microscopy (cryo-EM) is a revolutionary tool for studying the molecular architectures of proteins, viruses, cells and the specialized molecular machines within cells. To learn more about how cryo-EM works, from preparing samples to capturing atomic images and using them to help create treatments for disease. | SLAC National Accelerator Laboratory
What's cryo-EM? Cryogenic electron microscopy (cryo-EM) is a revolutionary tool for studying the molecular architectures of proteins, viruses, cells and the specialized molecular machines within cells. To learn more about how cryo-EM works, from preparing samples to capturing atomic images and using them to help create treatments for disease. | SLAC National Accelerator Laboratory
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A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion
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What's cryo-EM? Cryogenic electron microscopy (cryo-EM) is a revolutionary tool for studying the molecular architectures of proteins, viruses, cells and the specialized molecular machines within cells. To learn more about how cryo-EM works, from preparing samples to capturing atomic images and using them to help create treatments for disease. | SLAC National Accelerator Laboratory
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Using focused ion beam scanning electron microscopy (FIB-SEM), samples are imaged with the SEM before a focused ion beam mills away as little as 3-10 nm. The sample is then imaged again and the process is repeated to build up a high-resolution view of the complete 3D volume. Cryogenic FIB-SEM generates these volumes from vitrified samples and enables the capture of 3D ultraresolution in the near native state. The video illustrates a typical cryogenic FIB-SEM workflow. Learn more about cryogenic
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Australian Vacuum Services on Instagram: "Cryogenic Electron Microscopy involves freezing organic material in slush liquid Nitrogen before cleaving, dehydration and deposition of a Pt, Au or Ir layer to take out the charging effects. Vacuum load locks and transfer sticks allow an all-vacuum workflow, before mounting inside the SEM. #cryo #microscooy #dehydration #sputtering #SEM"
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