The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...

Seroepidemiological studies have shown that approximately 60% of adults in developed countries and up to 100% of adults in developing countries are positive for antibodies against HCMV. 5 BMT ...

COVID-19 is commonly diagnosed by quantitative fluorescence real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) (1, 2). The test is the sequential action of two enzymes–an ...

Finding a COVID-19 test has become a real challenge with the spread of the omicron variant. But there is also confusion over which test people should be getting, which is a PCR or antigen? “The two ...

Using the procedure reported here, all the pipetting steps in LM-PCR can be performed on a Biomek 2000 liquid handling robotic workstation. After thermocycling is completed in the first reaction, the ...