A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all of the proteins associated with a particular tissue or ...

Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

An interview with Ramya Prabakaran & Melody Chang from GenScript, at SfN 2018, discussing the major challenges for researchers carrying out western blotting, and how GenScript's automated systems are ...

Western blot detection methods each have their own advantages and disadvantages. Choosing the right one can be a difficult decision. The basic protocol of the western blot – also referred to as the ...

Western blots have been a tried-and-true method for separating and quantifying proteins based on molecular weight, but they have not kept up with the continually increasing output demands of modern ...

The formation of new blood vessels by angiogenesis is important for tumor growth beyond 2 mm 3 in size. Over the past decade anti-angiogenic therapies that slow blood vessel growth, often by ...

Western blotting has, despite the initial unclear potential and its limitations, matured into an essential biochemical technique. This year marks the 40th anniversary of Western blotting [1]. Briefly, ...