Before setting a gating strategy, it is vital to know the properties of cells to be analyzed, such as cell size, the relative expression of markers and whether artifacts are likely to have been ...

Flow cytometry is broadly used in biomedicine, which is exemplified by identification of protein marker expressions 1,2,3,4,5,6, determinations of cell-fate and cell cycle progression 7, analysis of ...

Many common flow cytometry mistakes share a common feature—simply following procedures instead of cultivating conceptual ...

Differences in how users gate populations within experiments are major sources of variability in flow cytometry data analysis. Incorporating automated image analysis can substantially reduce user bias ...

A group from the Shanghai Jiao Tong University School of Medicine has outlined a protocol for the tissue-resident myeloid populations in mouse tissues using flow cytometry. The protocol builds on ...

Subjectivity in flow-cytometry data analysis has long been a challenge. Recent advancements in high-speed camera capabilities have led to the integration of morphological features with traditional ...

Flow cytometry remains a critical technology for the high-throughput analysis of single cells in complex populations. Attention to good analysis practices is more important than ever due to the recent ...

The Cytometry and Cell Sorting Facility at Baylor College of Medicine provides training, instrumentation, technical expertise, and software for flow cytometric analysis and cell sorting. This site ...

After five decades of use, flow cytometry is entrenched in biomedical science. Besides enabling the quick processing of cells in suspension, flow cytometry provides quantitative results across ...

Immune cells comprise a diverse and dynamic cell population that is responsible for a broad range of immunological activities. They act in concert with other immune and nonimmune cells via ...

Around the same time, Mack Fulwyler, an engineer working at Los Alamos National Laboratory, needed to separate particles, so he drew on existing techniques to create droplets to separate cells from a ...