Lysis buffer composition and protein lysate preparation is crucial for the success of RPPA. Discuss with the core director before preparing your lysates. View detailed protocols for lysate preparation ...
Cell or tissue lysates are arrayed on nitrocellulose-coated slides (Grace Bio-labs, Bend, OR) in triplicate using the Aushon 2470 Arrayer (Aushon BioSystems, Billerica, MA) (technical replicates).
We collected a unique pair of microRNA sequencing data sets for the same set of tumor samples; one data set was collected with and the other without uniform handling and balanced design. The former ...
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